Molecular Immuno-response Effects for Iraqi Lycium barbarm Carotenes upon Normal Human lymphocytes culture

Objectives: The study aimed to investigate the effects of Iraqi Lycium barbarm Carotene on normal human blood lymphocytes at molecular level.
 Methods: culture was exposed extracted from estimate interleukins expression in treated represented by IL-10 and TNF-α as a moderator immune response scale, their CD markers changes (CD3, CD4&CD8) flow-cytometer apparatus.
 Results: Total carotenes content 0.33mg/g powdered dried fruit. Potent carotene effect appeared concentration 500µg/ml for with increasing CD3 level get upper after 2 hours interval, while 4 exposure both CD4 CD8 levels increased dramatically 125 µg/ml, 250µg/ml carotene. An alteration cytokines gene production treatments (125,250,500) µg/ml intervals, suppress tumor necrosis factor (TNF-α) relation β-actin internal control PHA mitogenic agent.
 Conclusion: concluded that fruits seemed initiate system toward Th2 cell activation rather thanTh1, besides extract may potentiate production.